You are almost there: your candidate enzyme is highly active and performs well in development, but its thermostability still falls short of market requirements. In feed applications, insufficient thermal tolerance during processing can directly impact product performance, process robustness, and commercial scalability.

The challenge is well known: improving thermostability often comes at the cost of enzyme activity. Avoiding this trade-off is critical for successful product integration and manufacturing.

Aminoverse relies on experience in thermostability engineering without activity loss. Using targeted evolution strategies and high-throughput screening, we identify variants with improved thermal tolerance while maintaining (or even improving) catalytic performance. To support seamless process integration and scale-up, engineering can be performed directly in industrially relevant production hosts such as Pichia pastoris (Komagataella phaffii), instead of developing everything in E. coli only to learn later that improvements were not transferable.

For a customer in the feed industry, Aminoverse developed a thermostable enzyme variant optimized for industrial processing:

• Increased tolerable incubation temperature from 52 °C to >80 °C
• Achieved in only two rounds of evolution while maintaining full activity
• Engineering performed directly in Pichia pastoris to support commercial production
compatibility
• Delivered gram quantities for direct application tests (pelleting)
• Navigated crowded patent landscape to secure novel IP