Fe(II)- and α-ketoglutarate-dependent oxygenases (KGOs), also known as 2-oxoglutarate-dependent oxygenases (2OGs) or αKGDs, form a large and diverse family of non-heme iron enzymes that catalyze oxidative transformations using molecular oxygen and α-ketoglutarate (αKG) as co-substrate. Unlike complex P450 enzymes, KGOs are streamlined and efficient, with no intricate domain structures that can lead to instability. This means they are robust under diverse conditions, making them ideal for reliable, scalable applications in even the most demanding workflows.

KGOs, often derived from bacterial sources, are expressed intracellularly, where they influence native metabolic pathways by metabolite modification. These enzymes are renowned for catalyzing a variety of reactions, most prominently hydroxylation, but also halogenation, desaturation, and ring formation, all under mild aqueous conditions at ambient temperature and pressure. These enzymes only require α-ketoglutarate as oxygen donor which can be continuously supplemented or regenerated during the reaction via an enzymatic side reaction. This cofactor-based mechanism confers high stereo-, chemo-, and regioselectivity, enabling transformations that are chemically challenging through conventional synthesis.

Industrially, the scalability of KGO-catalyzed reactions has been demonstrated, with fermentation-based processes now capable of yielding up to 400 kilograms enzyme. Such scalability, combined with their operational mildness and catalytic precision, establishes KGOs as highly valuable biocatalysts for sustainable synthesis of fine chemicals, pharmaceuticals, and complex intermediates.

Identifying the right Fe(II)-α-ketoglutarate oxygenase for a given substrate remains a major challenge in biocatalytic research, where experimental screening often consumes time, material, and cost. Conventional enzyme panels frequently yield few or no active hits due to the limited substrate scope or incomplete enzyme representation.

Curated KGO kits address this gap by leveraging computational curation and predictive modeling to match enzyme reactivity with specific substrate classes. By integrating sequence homology searches, structural modeling, and active-site profiling, in silico selection enables the rational assembly of focused enzyme panels with improved hit probability. This approach significantly reduces the cost and duration of experimental discovery, as only the most promising candidates are expressed and tested.

Panels dedicated to particular substrate categories, such as amino acids, nucleic acids, or heterocyclic scaffolds, allow rapid identification of functional biocatalysts and facilitate reaction optimization in early development. Curated kits thus streamline biocatalyst discovery while maintaining high predictive accuracy, accelerating the path from screening to scalable synthesis.

The AminoAcid KGO Panel is designed to give you a head start in harnessing the power of curated ketoglutarate oxygenases (KGOs) for amino acid innovation. With this panel of 42 KGOs, you gain access to enzymes that enable the tailored functionalization of amino acid derivatives, opening the door to advanced building blocks, peptide-based materials, and even rare or unnatural amino acids with direct relevance for pharmaceutical and biotech applications. Potenital products are trans-3-hydroxy-L-proline, 4-chloride-lysine, cis-3-hydroxy-L-pipecolic acid, or 5-hydroxy-leucine.

For R&D teams, the AminoAcid Panel offers a faster route to discovery. Each enzyme in the collection has been carefully pre-selected for substrate compatibility, ensuring higher hit rates and reducing time spent on trial-and-error screening. By unlocking transformations that are difficult—or even impossible—with traditional chemical methods, the panel creates unique opportunities for innovation in drug development and beyond.

Whether your goal is to design next-generation peptide APIs, develop functional biomaterials, or explore novel molecular scaffolds, the AminoAcid KGO Panel provides a direct pathway to high-probability discovery in the biocatalytic oxyfunctionalization of both canonical and non-canonical amino acids.

The BioCarbon KGO Panel empowers you to explore the full potential of ketoglutarate oxygenases (KGOs) in transforming carbon-based natural products. With this curated panel of 38 KGOs, you can create novel flavors and fragrances, develop bioactive agrochemicals, modify hormones, and synthesize valuable drug precursors—all through precise biocatalytic oxyfunctionalization.

Designed for innovation in Flavours & Fragrances and related markets, the BioCarbon Panel accelerates discovery by enabling reactions that are challenging or impossible using conventional chemistry. Each enzyme has been carefully selected for substrate compatibility, increasing your chances of success while reducing the time spent on screening.

Whether you are optimizing natural product scaffolds, exploring new functionalities, or developing high-value compounds, the BioCarbon KGO Panel provides a streamlined path to high-probability discovery in the biocatalytic modification of fatty acids, terpenes, alkaloids, and steroids.

The XNA KGO Panel provides a curated set of ketoglutarate oxygenases (KGOs) designed to unlock site-selective modifications of nucleosides and nucleotides. With this panel of 22 KGOs, you gain access to novel nucleic acid derivatives, functionalized oligonucleotides for therapeutic applications, and advanced tools for synthetic biology and molecular probe development. Potential products are hydroxy-methyluracil, 5-methyluridine, 5-formyluracil, or 2′-Chloro-2′-deoxyadenosine 5′-monophosphate.

By leveraging this ready-to-screen panel, R&D teams can accelerate discovery while performing transformations that are challenging or inaccessible through traditional chemistry. Each enzyme has been carefully curated for targeted substrate compatibility, maximizing hit rates and minimizing trial-and-error experimentation.

Whether your focus is on advancing RNA therapeutics, exploring DNA modification tools, or engineering unique nucleic acid derivatives, the XNA KGO Panel provides a streamlined pathway to high-probability discovery in nucleic acid biocatalysis.

The Eureka Kit is our most comprehensive KGO collection, bringing together 41 KGO enzymes mined from sequence space and refined through advanced in silico prediction. By integrating structural modeling with patent landscaping, this panel not only maximizes your chances of functional hits but also strengthens your intellectual property position.

Building on these foundations, the Eureka Panel extends into broader applications, from antibiotic development to the modification of diverse and complex substrates. It enables precise transformations beyond the reach of conventional chemistry, giving you faster access to high-value derivatives and increasing your chances of patentable discoveries.

With the Eureka KGO Panel, you can explore new synthesis routes with greater speed, reduced risk, and a higher probability of success—making it the ultimate shortcut to discovery across pharma, biotech, and beyond.